黄色ブドウ球菌の産生する Panton-Valentine Leukocidin 検出用試薬，
宮 城 郁 乃*1 仲宗根 勇*2 山 根 誠 久*3
Laboratory-Based Evaluation of PVL-RPLA “Seiken”
to Detect Panton-Valentine Leukocidin Produced by Staphylococcus aureus
Ayano MIYAGI*1, Isamu NAKASONE*2 and Nobuhisa YAMANE, MD, PhD*3
It is well known that some isolates of Staphylococcus aureus produce pathogenic toxin, Panton-Valentine
leukocidin(PVL), and that the toxin has been reported to be highly associated with community acquiredmethicillin resistant S. aureus(CA-MRSA). Currently, the PCR method using specific primers for the PVL
gene(LukS-PV-lukF-PV) have been widely used to detect PVL. In this study, we evaluated the PVL-RPLA
“Seiken”, diagnostic reagent based on a reserved passive latex agglutination reaction with a specific monoclonal
antibody for detecting PVL. A total of 630 clinical isolates were used. PCR method detected 34
PVL-positive(28 MRSA and 6 MSSA), and, of these, PVL-RPLA “Seiken” read positive for 32 isolates(27
MRSA and 5 MSSA), the result indicating two false negative occurrences. The concordance rate was 99.7%.
In addition the recommended BHI broth, CCY medium, Dolman broth and Todd-Hewitt broth were applied
for toxin preparation media. Toxin concentration produced in CCY medium was significantly higher than
those in the remaining culture medium(p＜0.05).
PVL-RPLA “Seiken” is a method for detecting the PVL in the culture broth by antigen-antibody reaction
after an overnight shaking culture. This method does not require any expensive equipments or facilities.
Thus this reagent provides us with rapid, easy-to-perform, less expensive test method to detect PVL in clinical
[Rinsho Byori 62 : 533～537, 2014]
Corresponding author: Ayano MIYAGI, Clinical Laboratories, University Hospital of the Ryukyus, Nakagamigun,
Okinawa-pref. 903-0215, Japan. E-mail: [email protected]
【Key Words】Panton-Valentine leukocidin: PVL(パントン・バレンタイン・ロイコシジン)，reversed passive
latex agglutination: RPLA(逆受身ラテックス沈降凝集反応)，methicillin-resistant Staphylococcus aureus: MRSA
(メチシリン耐性黄色ブドウ球菌)，community-acquired MRSA: CA-MRSA(市中感染型 MRSA)