Ciliary Neurotrophic Factor

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Rev 082675A
Ciliary Neurotrophic Factor
Human, Recombinant (rHuCNTF)
Expressed in E. coli
Cat. No. CRP0876
Lot. No. (See product label)
Description: Ciliary neurotrophic factor (CNTF) is a
polypeptide initially purified from chick embryo ocular tissue
and identified as a trophic factor for embryonic chick ciliary
parasympathetic neurons in culture. Subsequent studies
have demonstrated that CNTF is a survival factor for
additional neuronal cell types including: dorsal root ganglion
sensory neurons, sympathetic ganglion neurons, embryonic
motor neurons, major pelvic ganglion neurons and
hippocampal neurons. CNTF has also been shown to
prevent the degeneration of motor axons after axotomy.
Amino-Acid Sequence: 199 aa, non-glycosylated
PDB rendering based on 1cnt.
Gene Name: CNTF
M. W. : 22,800 Da
Synonyms: HCNTF,
neurotrophic factor
Recombinant: Expressed in E. coli
Purity: >97% by SDS-PAGE and HPLC analyses.
Formulation: Lyophilized from a 0.2mm
concentrated (1mg/ml) solution in PBS, pH 7.4.
Biological Activity:
Fully biologically active when
compared to standard. The ED50 as determined by the
dose-dependant stimulation of TF-1 cells is less than 3.0 ng/
Endotoxin: Less than 1EU/mg of rHuCNTF as determined
by LAL method.
Reconstitution: We recommend that this vial be briefly
centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous
buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/
ml. Stock solutions should be apportioned into working
aliquots and stored at <-20°C. Further dilutions should be
made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable for several
weeks at 2-8°C, but should be kept at –20°C for long term
storage, preferably desiccated. Upon reconstitution, the
preparation is stable for up to one week at 2-8°C. For
maximal stability, apportion the reconstituted preparation into
working aliquots and store at –20° C to –70° C. Avoid
repeated freeze/thaw cycles.
mRNA Refseq: NM_000614.2
Protein Refseq: NP_000605.1
MIM: 118945
GeneID: 1270
Uniprot ID: P26441
Chromosome Location: 11q12.2
Pathway: Cytokine-cytokine receptor interaction, Jak-STAT
signaling pathway
Function: ciliary neurotrophic factor receptor binding, growth
factor activity, protein binding.
1. Sendtner M, Carroll P, Holtmann B, et al. Ciliary
neurotrophic factor. J. Neurobiol. 1995; 25 (11): 1436–1453.
2. Sleeman MW, Anderson KD, Lambert PD, et al. The ciliary
neurotrophic factor and its receptor, CNTFR alpha.
Pharmaceutica acta Helvetiae 2000; 74 (2-3): 265–272.
3. Schooltink H, Stoyan T, Roeb E, et al. Ciliary neurotrophic
factor induces acute-phase protein expression in
hepatocytes. FEBS Lett. 1993; 314 (3): 280–284.
4. Bazan JF. Neuropoietic cytokines in the hematopoietic
fold. Neuron 1991; 7 (2): 197–208.
@ 2005-2008 Creative Biolabs. All rights reserved.
21 Brookhaven BLVD ·Port Jefferson Station, NY 11776, USA
Technical Support: T: 631-871-5806 ·F: 631-207-8356
E-mail: [email protected]

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