Rabbit Anti-Human Luteinizing Hormone (LH ) Polyclonal Antibody

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E1748RUO
Rev E
Page 1 of 1
Rabbit Anti-Human Luteinizing Hormone (LH ) Polyclonal Antibody
E17480
0.1 ml immunogen affinity purified rabbit polyclonal
antibody in PBS/1% BSA buffer pH 7.6 with less than
0.1% sodium azide.
E17482
0.5 ml immunogen affinity purified rabbit polyclonal
antibody in PBS/1% BSA buffer pH 7.6 with less than
0.1% sodium azide.
E17484
1.0 ml immunogen affinity purified rabbit polyclonal
antibody in PBS/1% BSA buffer pH 7.6 with less than
0.1% sodium azide.
CATALOG #:
E17481
7.0 ml pre-diluted immunogen affinity purified rabbit
polyclonal antibody in TBS/1% BSA buffer pH 7.6 with
less than 0.1% sodium azide.
Human anterior pituitary
stained with anti-luteinizing
hormone (LH) antibody
INTENDED USE:
For Research Use Only. Not for use in diagnostic procedures.
CLONE:
N/A
IMMUNOGEN:
Purified human luteinizing hormone.
IG ISOTYPE:
Rabbit IgG
EPITOPE:
Not determined
MOLECULAR WEIGHT
28kDa
SPECIES REACTIVITY:
Human (tested). (See www.springbio.com for information on species reactivity predicted by
sequence homology.)
DESCRIPTION:
LH is a tropic hormone which modulates the secretory activity of other endocrine glands. It is
produced in the anterior hypophysis of the pituitary gland. The glycoprotein hormone, LH, like
follicle stimulating hormone and thyroid stimulating hormone, is composed of a common alphasubunit but also a specific beta-subunit, which characterizes each of these hormones.
APPLICATIONS:
Immunohistochemistry (IHC)
IHC PROCEDURE:
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this
primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:500. The
dilutions are estimates; actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by
cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the
visualization system.
POSITIVE CONTROL:
Anterior pituitary
CELLULAR LOCALIZATION:
Cytoplasm
STORAGE & STABILITY
Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When
properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent
beyond the expiration date.
There are no definitive signs to indicate instability of this product; therefore, positive and negative
controls should be tested simultaneously with unknown specimens.
If unexpected results are observed which cannot be explained by variations in laboratory
procedures and a problem with the reagent is suspected, contact Technical Support at
[email protected]
WARNINGS & PRECAUTIONS:
1.
2.
3.
4.
4300 Hacienda Drive
Pleasanton, CA 94588
www.springbio.com
Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact
with sensitive areas, wash with copious amounts of water.
This product is harmful if swallowed.
Consult local or state authorities with regard to recommended method of disposal.
Avoid microbial contamination of reagents.
Tel: 1-925-474-8440
Toll Free: 1-800-787-6896
Fax: 1-925-474-8469
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