BD Vacutainer® - St Vincent`s University Hospital

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BD Vacutainer
®
BD Diagnostics - Preanalytical Systems
Tube Guide & Recommended Order of Draw*
* Clinical and Laboratory Standards Institute (Formerly NCCLS) Guidelines H3-A6, 6th Edition
St Vincents University Hospital, Dublin
Blood samples should be taken in the following order:
Cap Colour
Cat. No.
Additive
Determinations
Special instructions
Blood
Culture
If insufficient blood for both culture bottles, use aerobic bottle only. Using a needle-protected butterfly
needle collect aerobic (green) bottle first and then the anaerobic (purple) bottle. If using a syringe then the
anaerobic bottle is taken first and then the aerobic bottle.
Blood Culture bottles should be available on all wards. A supply is available from Microbiology Reception
(Mon – Fri)
Sodium
Citrate
Coagulation Testing, PT, INR, APTT, D-Dimer, Thrombophilia
Screens, Factor Assays
3-4
Serum
LDH, NT-pro BNP, Drugs (Lithium, Phenytoin, Theophylline,
Methotrexate, Valproate, Carbamazepine, Paracetamol,
Salicylate, Digoxin, Tobramycin).
Ionised Calcium, Vitamin D, Parathyroid Hormone, Osmolality,
Bone Markers, Endocrine Testing (excluding Thyroid).
5-6
Serum/Gel
Growth Hormone, ADNA, Gastrin, Electrophoresis,
Immunoglobins (IgG, IgM,IgA,IgE), B2 Microglobin,
Ceruloplasmin.
Infectious Mono,Thyroid Ab, Liver Ab, Rheumatology, Coeliac
Ab
5-6
Plasma/Gel
UE, LFT, Creatine Kinase, Ca, Mg, Phosphate, Uric Acid, Total
Protein, Amylase, Lipids, Bone Profile, Troponin T, Iron Studies,
ACE, CRP, TSH, FT4, T3, Cortisol, Vitamin B12, Folate, Ferritin,
PSA, Free PSA, CEA, AFP,HCG,CA125, CA19.9,CA153
Cat. No.
363095 / 363093
Draw Volume
Mix
Times
2.7ml / 1.8ml
Cat. No.
367837
Draw Volume
6ml
Cat. No.
367954
Draw Volume
5ml
Cat. No.
367375
Draw Volume
4.5ml
Cat. No.
368857
Draw Volume
EDTA
(Potassium)
Full Blood Count (FBC), ESR, C3/C4, Haemoglobin
A1c,Homocysteine, ACTH, Sirolimus, Tacrolimus, Cyclosporin,
PCR for CMV, HIV,EBV and Haemochromatosis Genetics.
3ml
Mix
Times
Mix
Times
Mix
One full plasma gel tube is sufficient
for all the tests in this group
8-10
Homocysteine and ACTH should be
taken onto ice. State time specimen
taken. One tube for FBC and ESR.
Separate tubes for each of the other
tests in this group
8-10
Times
Mix
Times
Cat. No.
Mix
Draw Volume
EDTA
Group; Crossmatch
Fluoride
Oxalate
Glucose and Ethanol
8-10
Times
Cat. No.
368920
Draw Volume
Mix
8-10
Times
2ml
Cat. No.
368380 / 368381
Draw Volume
Trace
Element
Zinc, Copper and Selenium - Navy Cap with a red strip on the
side of the label.
Chromium and Cobalt – Potassium EDTA Navy cap with blue
band on tube
Mix
8-10
Times
6ml
Mix
IMPORTANT MIXING GUIDELINES 8-10
Times
All BD Vacutainer tubes require immediate mixing following
collection. Insufficient mixing can result in inaccurate test results
and the need to re-draw. Correct mixing technique is to invert
each tube by the recommended number of times shown on the
right hand side of the table.
®
BD, BD Logo, Vacutainer and Hemogard are trademarks of Becton, Dickinson and Company ©2012 BD.
BD Diagnostics - Preanalytical Systems
The Danby Building
Edmund Halley Road
Oxford Science Park
Oxford OX4 4DQ
Tel: 01865 781603
Fax: 01865 781528

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