TASSEL 3.0 / 4.0 Pipeline Command Line Interface: Guide to using

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TASSEL 3.0 / 4.0 Pipeline Command Line Interface: Guide to using Tassel Pipeline
Terry Casstevens ([email protected])
Institute for Genomic Diversity, Cornell University, Ithaca, NY 14853-2703
March 28, 2014
Prerequisites ............................................................................................................................................................ 1 Source Code ............................................................................................................................................................ 1 Install....................................................................................................................................................................... 1 Execute.................................................................................................................................................................... 1 Increasing Heap Size............................................................................................................................................... 2 Examples ................................................................................................................................................................. 2 Examples (XML Configuration Files) .................................................................................................................... 2 Usage....................................................................................................................................................................... 3 Pipeline Controls................................................................................................................................................. 3 Data ..................................................................................................................................................................... 3 Analysis............................................................................................................................................................... 8 Results ............................................................................................................................................................... 10 Prerequisites
•
Java SDK 6.0 or later (http://java.sun.com/javase/downloads/index.jsp).
•
Tassel Standalone Build (http://www.maizegenetics.net/tassel/tassel3.0_standalone.zip)
•
Tassel Standalone Build (http://www.maizegenetics.net/tassel/tassel4.0_standalone.zip)
Source Code
•
https://tassel.svn.sourceforge.net/svnroot/tassel/maizegenetics
Install
Unzip the Tassel Standalone Build onto your file system. Change into the root directory:
tassel3.0_standalone or tassel4.0_standalone.
Execute
On Windows, use run_pipeline.bat to execute the pipeline.
In UNIX, use run_pipeline.pl to execute the pipeline. If you are using a Bash Shell on Windows, you
may need to change the following line to use a ; instead of a :.
my $CP = join(":", @fl);
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To launch the Tassel GUI that automatically executes a pipeline, use start_tassel.bat or
start_tassel.pl instead of run_pipeline.bat or run_pipeline.pl respectively.
These scripts have a $top variable that can be changed to the absolute path of your installation. That way, you
can execute them any directory.
Increasing Heap Size
To modify the initial or maximum heap size available to the Tassel Pipeline, either edit run_pipeline.pl
or specify values via the command line.
./run_pipeline.pl -Xms512m -Xmx10g -fork1…
Examples
./run_pipeline.pl -fork1 -h chr1_5000sites.txt -ld -ldd png -o
chr1_5000sites_ld.png -runfork1
./run_pipeline.pl -fork1 -h chr1_5000sites.txt -ld -ldd png -o
chr1_5000sites_ld.png -runfork1
./run_pipeline.pl -fork1 … -fork2 … -combine3 -input1 -input2 … -fork4 <flag> -input3 -runfork1 -runfork2
Examples (XML Configuration Files)
This command runs the Tassel Pipeline according to the specified configuration file... Configuration files are
standard XML notation. The tags are the same as the below documented flags although no beginning dash is
used. See the example_pipelines directory for some common XML configurations.
./run_pipeline.pl -configFile config.xml
This command creates the XML configuration file from the original command line flags. Simply insert the createXML and filename at the beginning. Only the XML is created. It does not run the pipeline...
./run_pipeline.pl -createXML config.xml -fork1 ...
This command translates the specified XML configuration file back into the original command line
flags... It does not run the pipeline...
./run_pipeline.pl -translateXML config.xml
2
Usage
Pipeline Controls
-fork<id>
-runfork<id>
-input<id>
-inputOnce<id>
-combine<id>
This flag identifies the start of a pipeline
segment that should be executed sequentially.
<id> can be numbers or characters (no spaces).
No space between -fork and <id> either. Other
flags can reference the <id>.
This flag identifies a pipeline segment to
execute. This will usually be the last argument.
This explicitly executes the identified pipeline
segment. This should not be used to execute
pipeline segments that receive input from other
pipeline segments. Those will start
automatically when it receives the input.
This specifies a pipeline segment as input to
the plugin prior this flag. That plugin must be
in the current pipeline segment. Multiple of
these can be specified after plugins that accept
multiple inputs.
./run_pipeline.pl -fork1 -h genotype.hmp.txt fork2 -r phenotype.txt -combine3 -input1 -input2
-intersect -runfork1 -runfork2
./run_pipeline.pl -fork1 -h genotype.hmp.txt fork2 -includeTaxaInFile taxaList1.txt -input1 export file1 -fork3 -includeTaxaInFile
taxaList2.txt -input1 -export file2 -runfork1
This specifies a pipeline segment as a one-time
input to a CombineDataSetsPlugin. As such, this
flag should follow a CombineDataSetsPlugin in
the current pipeline segment. After the
CombineDataSetsPlugin has received data from
this input, it will use it for every iteration.
Whereas CombineDataSetsPlugin waits for data
specified by -input each iteration. Multiple of
these can be specified.
This flag starts a new pipeline segment with a
CombineDataSetsPlugin at the beginning. The
CombineDataSetsPlugin is used to combine data
sets from multiple pipeline segments. Follow
this flag with -input<id> and/or -inputOnce<id>
flags to specify which pipeline segments should
be combined.
Data
-t <trait file>
If the filename to be imported begins with
“http”, it will be treated as an URL.
Loads trait file as numerical data.
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-s <SNP file>
-p <Poly file>
-a <Anno file>
-r <phenotype file>
-k <kinship file>
-q <population
structure file>
-h <hapmap file>
-h5 <HDF5 file>
-b <BLOB file>
-g <BLOB file>
-plink -ped <ped
filename> -map <map
filename>
-flapjack -geno <geno
file> -map <map file>
-fasta <filename>
-geneticMap <filename>
-table
-vcf <filename>
-importGuess <filename>
Loads SNP file as sequence alignment.
Loads polymorphism file as polymorphism
alignment.
Loads annotated alignment file.
Loads file as Table Report. This is meant to
replace numerical, polymorphism, and annotated
alignment.
Loads kinship file as square matrix.
Loads population structure file as numerical
data.
Loads hapmap file (.hmp.txt or .hmp.txt.gz)
Loads HDF5 Alignment file (.hmp.h5).
Loads BLOB file (.zip). Only Tassel 3.
Loads BLOB file (.gz). Only Tassel 3.
Loads Plink format given ped and map files.
Loads Flapjack format given geno and map files.
Loads FASTA file.
Loads Genetic Map.
Loads a Table (i.e. exported from LD, MLM).
Loads VCF file.
Uses Tassel Guess function to load file.
-maxAllelesToRetain
<number>
Sets the preference maximum number of alleles to
retain. Initial default is 2. Notice this is a
preference setting and remains true on a given
machine for all import operations that follow,
even in subsequent executions of the Tassel
Pipeline or Tassel GUI.
-retainRareAlleles true Sets the preference whether to retain rare
| false
alleles. Notice this is a preference setting and
remains true on a given machine for all import
operations that follow, even in subsequent
executions of the Tassel Pipeline or Tassel GUI.
-optimizeForTaxa
This should follow a file import, which
instructs the loader to optimize the data for
taxa based operations. By default, loaded data
is optimized for site operations.
-convertToSiteOpt
-convertToTaxaOpt
Converts input Alignment to a Site Optimized
Alignment. May return same Alignment if already
optimized for Sites. This is mainly for testing
purposes.
Converts input Alignment to a Taxa Optimized
Alignment. May return same Alignment if already
optimized for Taxa. This is mainly for testing
purposes.
4
-taxaJoinStrict <type>
If type Strict (deprecated value true), taxa
names are only considered the same if they match
exactly. If type NonStrict (deprecated value
false), taxa names match if all specified levels
match. For example… B73 matches B73:XXX matches
B73:XXX:YYY. But B73:XXX does not match B73:YYY.
If type NumLevels, then taxa names match if
specified number of levels (-taxaJoinNumLevels)
match. For example… B73:XXX:YYY matches
B73:XXX:ZZZ for 1 or 2 number of levels.
-taxaJoinNumLevels
Specifies number of levels to use when
<num>
-taxaJoinStrict is NumLevels.
-union
This joins (union) input datasets based taxa.
This should follow a -combine specification.
-intersect
This joins (intersect) input datasets based
taxa. This should follow a -combine
specification.
-separate
This separates an input into its components if
<chromosomes…>
possible. For example, alignments separated by
chromosome (locus). For alignments, optionally
specify list of chromosomes (separated by commas
and no spaces) to separate. Specifying nothing
returns all chromosomes. Example:
run_pipeline.pl -fork1 -h file.hmp.txt -separate
3,6 -export -runfork1
-mergeAlignments
Merges multiple Alignments regardless of taxa or
site name overlap. Undefined taxa / sites are
set to UNKNOWN. Duplicate taxon / site set to
last Alignment processed. Example:
run_pipeline.pl -fork1 -h file1.hmp.txt -fork2 h file2.hmp.txt -combine3 -input1 -input2 mergeAlignments -export files_merged.hmp.txt runfork1 -runfork2
-mergeAlignmentsSameSites Merges Alignments assuming all sites are the
-input <files> -output
same in all Hapmap files. Input files separated
<filename>
by commas without spaces. The resulting file
may have incorrect major/minor alleles, strand,
center, etc. It uses values from first
specified input file. Checks that Site Name,
Chromosome, and Physical Position match for each
site. Example: run_pipeline.pl -fork1 mergeAlignmentsSameSites -input
file1.hmp.txt,file2.hmp.txt -output temp runfork1
-export
Exports input dataset to specified filename(s).
<filename1,filename2,…> If no -exportType follows this parameter, the
exported format will be determined by the type
of input (i.e. Alignments will default to Hapmap
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-exportType <type>
-impute
-imputeMethod <method>
-imputeMinLength <num>
-imputeMaxMismatch
<num>
-imputeMinProb <num>
format). Exportable datasets, other that
Alignment, only have one format option.
Therefore, there is no need to specify exportType. Specify none, one, or multiple
filenames matching the number of input data
sets. If no filenames, the files will be named
the same as the input data sets. If only one
specified for multiple data sets, a count
starting with 1 will be added to each resulting
file. If multiple filenames (separated with
commas but no spaces), there should be one for
each input. When exporting Hapmap files, if the
extension is .hmp.txt.gz, the file will be
gzipped.
Defines format that previously specified -export
should use. Type can be Hapmap, HapmapDiploid,
HDF5, VCF, Plink, zipBLOB (only Tassel 3),
gzipBLOB (only Tassel 3), Flapjack, Phylip_Seq,
Phylip_Inter, Text.
Imputes Genotypic Data.
Specifies the impute method to use. Method can
be Length (default), MajorAllele, SimilarWindow,
or IBDProb. This should follow the -impute flag.
Specifies the minimum length for the impute
Length method (default value: 30). This should
follow the -impute flag.
Specifies the maximum mismatch for the impute
Length method (default value: 1). This should
follow the -impute flag.
Specifies the minimum probability for the impute
IBDProb method (default value: 0.001). This
should follow the -impute flag.
-filterAlign
-filterAlignMinCount
<num>
-filterAlignMinFreq
<num>
-filterAlignMaxFreq
Filters an alignment by sites.
Specifies the minimum count (default: 1) for the
previously specified -filterAlign.
Specifies the minimum frequency (default: 0.0)
for the previously specified -filterAlign.
Specifies the maximum frequency (default 1.0)
for the previously specified -filterAlign.
-filterAlignStart <num> Specifies the starting site index (default
value: 0) for the previously specified filterAlign.
-filterAlignEnd <num>
Specifies the end site index (default value:
last site in alignment) for the previously
specified -filterAlign.
-filterAlignLocus
Specifies the Locus to be used with the starting
<name>
and ending physical positions if defined.
Defaults to first Locus in the Alignment.
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-filterAlignStartPos
<num>
-filterAlignEndPos
<num>
-filterAlignExtInd
-filterAlignRemMinor
-filterAlignSliding
-filterAlignHapLen
<num>
-filterAlignStepLen
<num>
-includeTaxa
<taxon1,taxon2,…>
-includeTaxaInFile
<filename>
-excludeTaxa
<taxon1,taxon2,…>
-excludeTaxaInFile
<filename>
Specifies the starting physical position
(default is first site) for the previously
specified -filterAlign.
Specifies the end physical position (default is
last site) for the previously specified filterAlign.
Indicates that the last specified -filterAlign
should extract indels. This is not done by
default.
Indicates that the last specified -filterAlign
should remove minor SNP states. This is not done
by default.
Indicates that the last specified -filterAlign
should use sliding windows. This in not done by
default.
Specifies the haplotype length (default value:
3) if using sliding windows.
Specifies the step length (default value: 3) if
using sliding windows.
Filters input alignment to only include
specified taxa. The taxa should be separated
with commas and no spaces.
Filters input alignment to only include taxa
specified in file. The taxa cannot have spaces.
Individual taxa should be separated by white
space.
Filters input alignment to exclude specified
taxa. The taxa should be separated with commas
and no spaces.
Filters input alignment to exclude taxa
specified in file. The taxa cannot have spaces.
Individual taxa should be separated by white
space.
-includeSiteNames
Filters input alignment to only include
<siteName1,siteName2,…> specified site names. The site names should be
separated with commas and no spaces.
-includeSiteNamesInFile Filters input alignment to only include site
<filename>
names specified in file. The site names cannot
have spaces. Individual site names should be
separated by white space.
-excludeSiteNames
Filters input alignment to exclude specified
<taxon1,taxon2,…>
site names. The site names should be separated
with commas and no spaces.
-excludeSiteNamesInFile Filters input alignment to exclude site names
<filename>
specified in file. The site names cannot have
spaces. Individual site names should be
separated by white space.
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-excludeLastTrait
This removes last column of Phenotype data. For
example… Can be used to remove last column of
population structure for use with MLM or GLM.
-subsetSites <num>
This filters an alignment to include a random
subset of sites. If <num> is >=1, it specifies
the total number of sites to keep. If it is a
decimal, it specifies the fraction of sites to
keep. Adding the flag "-step" immediately after
<num> tells the plugin to space the selected
sites evenly instead of randomly.
This filters an alignment to include a random
subset of taxa. If <num> is >=1, it specifies
the total number of taxa to keep. If it is a
decimal, it specifies the fraction of taxa to
keep. Adding flag "-step" immediately after
<num> tells the plugin to space the selected
taxa evenly instead of randomly.
-subsetTaxa <num>
-step
This tells the previously specified -subsetTaxa
or -subsetSites plugin to select sites/taxa
evenly across the alignment instead of randomly.
-homozygous
This function converts all heterozygous values
to Unknown. Example: ./run_pipeline.pl -fork1 h mdp_genotype.hmp.txt -homozygous -export
homozygous.hmp.txt -runfork1
-numericalGenoTransform Performs genotype to numerical transform. <type>
<type>
can be collapse or separated.
-newCoordinates <map
filename>
This converts alignment to new coordinates
specified in given map file.
-synonymizer
Runs the Synonymizer using the input dataset.
Analysis
-glm
-glmOutputFile
<filename>
-glmMaxP <number>
This takes a Phenotype dataset as input that is
usually the intersection of sequence data, trait
data, and population structure (optional).
This sends GLM results to specified filename.
-glmPermutations
<number>
This restricts the output file to entries with P
values no larger than number specified.
This sets the number of permutations. Default is
to not do run permutations.
-mlm
This takes a Phenotype dataset as input (usually
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the intersection of sequence data, trait data,
and population structure (optional)) and a
Kinship matrix.
-mlmVarCompEst <method> Defines the Variance Component Estimation for
the previously specified -mlm. Method can be P3D
(default) or EachMarker.
-mlmCompressionLevel
Defines the Compression Level for the previously
<level>
specified -mlm. Level can be Optimum (default),
Custom, or None.
-mlmCustomCompression
This specifies the compression when compression
<number>
level is Custom. Default value is 1.0.
-mlmOutputFile
This sends MLM results to specified filename.
<filename>
-mlmMaxP <number>
This restricts the output file to entries with P
values no larger than number specified.
-diversity
-diversityStartBase
<number>
-diversityEndBase
<number>
-diversitySlidingWin
-diversitySlidingWinStep
<number>
-diversitySlidingWinSize
<number>
Creates a Diversity Analysis step that uses an
Alignment as input
This sets start base for the previously
specified -diversity. Default is 0.
This sets end base for the previously specified
-diversity. Default is last site.
This uses sliding window analysis for the
previously specified -diversity.
This sets the sliding window step size for the
previously specified -diversity. Default is 100.
This sets the sliding window size for the
previously specified -diversity. Default is 500.
-ld
Creates LinkageDisequilibriumPlugin. Uses
Alignment from previous step to analysis linkage
disequilibrium.
-ldPermNum <number>
This sets permutation number for the previously
specified -ld. Default is 1000.
-ldRapidAnalysis true | Sets whether to use rapid analysis for the
false
previously specified -ld. Default is true.
-ldWinSize <number>
Sets the window size for the previously
specified -ld. Default is 50.
-ldType <type>
Sets the LD type for the previously specified ld. Options are All, SlidingWindow (Default),
and SiteByAll.
-ldTestSite <number>
Sets the test site for when LD type is set to
SiteByAll.
-ldHetTreatment <type> Sets the LD Heterzygous Treatment Method. Type
can be Haplotype (Default - For Inbred Lines),
Homozygous (Uses only homozygous site heterozygotes set to missing), or Genotype (Not
Implemented Yet).
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-ck
-ckModelHets <type>
Calculates Kinship from Marker Data.
Sets how to model heterozygotes. Choose default
type RelateHomo (Related to Homozygotes) or
IndepState (Independent allele state).
-ckRescale true | false Set whether to rescale results between 2 and 0.
Default is true.
-tree <clustering
method>
-treeSaveDistance true
| false
This creates a tree using given clustering
method: Neighbor (default) or UPGMA. When
exporting, use -exportType Text to get text
version.
This saves the distance matrix of a tree.
Default is true.
-distanceMatrix
Calculate the distance matrix of given
Alignment.
-distMatrixRanges
Calculates genetic distances for given taxon in
specified physical position ranges.
Locus that specified physical positions
corresponds.
Taxon of interest.
-distMatrixRangesLocus
<locus>
-distMatrixRangesTaxon
<taxon>
-distMatrixRangesPos
<pos1,pos2,pos3,…>
-distMatrixRangesPosFile
<filename>
-gs
-genotypeSummary
<types>
Specified physical positions that define ranges.
A comma should separate each one with no spaces.
File with list of physical positions that define
ranges. Individual positions should be
separated by white space.
Predicts phenotypes using ridge regression for
genomic selection.
This generates summaries for alignment datasets.
Types should be a comma-separated list (with no
spaces) of the following (overall, site, taxa,
all). Example -genotypeSummary overall,site
Results
-td_csv <filename>
-td_tab <filename>
-td_gui
-ldd <output type>
Writes (comma delimited) TableReport from
previous plugin in current pipeline to specified
filename.
Writes (tab delimited) TableReport from previous
plugin in current pipeline to specified
filename.
Displays TableReport from previous plugin in
current pipeline in GUI.
Creates LinkageDiseqDisplayPlugin. If output
type is gui, this graphically displays results
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-ldplotsize <num>
-ldplotlabels true |
false
-o <output file>
from a LinkageDisequilibriumPlugin. If output
type is png, gif, bmp, jpg, or svg, then an
image of that type is written to the output file
specified with -o.
Optionally specify LD plot size. Example: 1000
will produce a 1000 x 1000 plot. Default: 500.
This should follow the -ldd flag within the
current pipeline segment.
Optionally specify whether to show the LD
Plot labels. DEFAULT: true. This should follow
the -ldd flag within the current pipeline
segment.
This should follow the -ldd flag within the
current pipeline segment.
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